Journal: Oncotarget

Article Title: Enhanced transfection efficiency and targeted delivery of self-assembling h-R3-dendriplexes in EGFR-overexpressing tumor cells

doi:

Figure Lengend Snippet: a. Internalization and subcellular localization of dendriplexes (N/P 20:1) and h-R3-dendriplexes (N/P 20:1, h-R3/DNA 1:1) in HepG2 cells. (green: LysoTracker Green used to label late endosomes and lysosomes; red: Cy5-labled PAMAM; yellow: colocalization). Scale bars = 5 μm. b. Intracellular localization of dendriplexes (N/P 20:1) and h-R3-dendriplexes (N/P 20:1, h-R3/DNA 1:1) in HepG2 cells. (red: Cy5-labled PAMAM; blue: Hoechst33342 stained cell nuclei; pink: colocalization). Scale bars = 5 μm.

Article Snippet: PAMAM was labeled with Cy5 mono-reactive NHS ester (Fanbo Biochemicals, Beijing, China).

Techniques: Staining

Journal: Oncotarget

Article Title: Enhanced transfection efficiency and targeted delivery of self-assembling h-R3-dendriplexes in EGFR-overexpressing tumor cells

doi:

Figure Lengend Snippet: a. Overlay images of X-ray and fluorescence imaging of three samples in vitro . b. Ex vivo fluorescence imaging of the tumor at 4 h, 12 h and 24 h after i.v. injection to HepG2 tumor-bearing nude mice. c. Region-of-interest analysis of fluorescent signals from the tumors 4 h, 12 h and 24 h after i.v. injection to mice. Error bars indicate s.d. ( n = 3). ** P < 0.01. d. Ex vivo fluorescence imaging of five major organs and tumor 24 h post-injection (T, tumor; H, heart; Lv, liver; S, spleen; Lu, lung; K, kidney). e. Region-of-interest analysis of fluorescent signals from the tumors and normal organs. (T, tumor; H, heart; Lv, liver; S, spleen; Lu, lung; K, kidney). Error bars indicate s.d. ( n = 3). *** P < 0.01. f. Ex vivo distribution of gene expression in the tumor 24 h after i.v. injection to nude mice-bearing HepG2 tumor. Frozen tumor sections (6 um thick) were examined by CLSM. Red homogeneous spots present the Cy5-dendriplexes and Cy5-h-R3-dendriplexes. Scale bars = 100 μm.

Article Snippet: PAMAM was labeled with Cy5 mono-reactive NHS ester (Fanbo Biochemicals, Beijing, China).

Techniques: Fluorescence, Imaging, In Vitro, Ex Vivo, Injection, Gene Expression

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA-Protein Complexes

doi: 10.1007/978-1-61779-005-8_26

Figure Lengend Snippet:

Article Snippet: For each labeling reaction prepare 15 μl of the dye solution, any remaining unused solution is unstable and it is preferable to prepare fresh solution for each set of labeling reactions. table ft1 table-wrap mode="anchored" t5 Cy3 Mono NHS Ester (766 Da) 15 mM ~11.5 mg/ml Cy5 Mono NHS Ester (792 Da) 15 mM ~11.9 mg/ml Oregon Green 488-X 1 NHS Ester (623 Da) 15 mM ~ 9.4 mg/ml Open in a separate window Combine the following in a foil wrapped or amber colored eppendorf.

Techniques: